KASP and STS markers associated with the chromosome 3B short arm translocation (T3BL.3NsbS) from Leymus racemosus

KASP markers were developed based on the genotyping results generated through DArTSeqTM and the AxiomTM Wheat Breeders Genotyping Array. Genotyping results included Leymus racemosus, several T3BL.3NsbS translocation lines in CIMMYT elite spring bread wheat genetic backgrounds, and other CIMMYT elite parents. KASP markers were designed based on the consensus sequence of DArTSeq and Axiom markers that could clearly distinguish the source and translocation lines from other CIMMYT elite parents. The KASP markers were selected to be bi-allelic and are currently used at CIMMYT for transferring translocations of Leymus racemosus in additional elite spring bread wheat backgrounds.